CRISPR/Cas9 ribonucleoprotein-mediated knockin generation in hTERT-RPE1 cells

CRISPR/Cas9 ribonucleoprotein-mediated knockin generation in hTERT-RPE1 cells

Blog Article

Summary: hTERT-RPE1 cells Shoe/Boot Covers are genetically stable near diploid cells widely used to model cell division, DNA repair, or ciliogenesis in a non-transformed context.However, poor transfectability and limited homology-directed repair capacity hamper their amenability to gene editing.Here, we describe a protocol for rapid and efficient generation Faber TWEET EG8 BK A55 55cm Wall Chimney Cooker Hood Extractor 330.0572.387 of diverse homozygous knockins.In contrast to other approaches, this strategy bypasses the need for molecular cloning.Our approach can also be applied to a variety of cell types including cancer and induced pluripotent stem cells (iPSCs).